Immunohistochemical Analysis of Apoptosis Markers

Tissue specimens from mice were formalin-fixed then subjected to IHC staining as described previously [27 (link),34 (link)]. Briefly, the tissue sections were de-waxed and rehydrated. The sections were stained with monoclonal antibodies against cleaved PARP and cleaved caspase3 (Cell Signaling) for 24 h at 4°C. After washing, the samples were probed with peroxidase-labeled goat anti-rabbit secondary antibody (Epitomics, Burlinggame, CA) and detected with an ABC kit (Vector Laboratories, Burlingame, CA).

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Chen Y.A., Lien H.M., Kao M.C., Lo U.G., Lin L.C., Lin C.J., Chang S.J., Chen C.C., Hsieh J.T., Lin H., Tang C.H, & Lai C.H. (2017). Sensitization of Radioresistant Prostate Cancer Cells by Resveratrol Isolated from Arachis hypogaea Stems. PLoS ONE, 12(1), e0169204.

Publication 2017

cleaved PARP and cleaved caspase3 peroxidase-labeled goat anti-rabbit secondary antibody ABC kit

Anti antibody Caspase3 Formalin Goat Mice Monoclonal antibodies Peroxidase Rabbit Tissue Vector

Corresponding Organization : Chang Gung Memorial Hospital

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Variable analysis

independent variables

Tissue specimens from mice

dependent variables

Immunohistochemical (IHC) staining for cleaved PARP and cleaved caspase3

control variables

Formalin-fixation of tissue specimens
Positive control: Monoclonal antibodies against cleaved PARP and cleaved caspase3
Negative control: Not explicitly mentioned

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