Kidney Transcriptome Profiling in TRACK Mice

High quality RNA was extracted from a thin layer of kidney cortex tissue (n = 6) using mini-RNAeasy columns (Qiagen) and RNAs were converted to cDNA by semi-quantitative reverse transcriptase (RT)-PCR. The WCMC Genomics Core performed the sequencing (51-bp single-end reads) on an Illumina HiSeq2000 Sequencer and data were analyzed as previously described [17 (link)]. In brief, data analysis was mainly performed with the Tuxedo tools software [23 (link)]. A stringent threshold was used to select differentially expressed genes (fold change >2 or <0.5, q<0.01). 655 genes showed increased mRNA levels and 55 genes showed decreased mRNA levels in TRACK TG+ kidney samples compared with the TG− control kidney cortex samples. Data are in the GEO database (accession no. GSE54390).