Neutrophil depletion with Ly6G antibody was carried out as previously described (30 (link)). Briefly, WT and IP6K1-deficient mice were intraperitoneally injected with a single dose of anti-mouse Ly6G antibody (400 μg/kg; clone 1A8, BioLegend). The antibody was administered intraperitoneally to obtain a sustained depletion over the first 48 hours of the experiment. Differential white blood cell count using Wright-Giemsa staining was performed to confirm that the neutrophil depletion was successful (peripheral blood neutrophil count was reduced by >85%) (30 (link)).
Protocol detail
Neutrophil Depletion Protocol Using Ly6G Antibody
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Anti antibody
Antibody
Clone
Giemsa
Ip6k1
Mouse
Neutrophil
Peripheral blood count
White blood cell count
Corresponding Organization : Institute of Hematology & Blood Diseases Hospital
Other organizations : Johns Hopkins Medicine, Johns Hopkins University, Veterans of Foreign Wars, Emory University
Top 5 similar protocols
Variable analysis
independent variables
- Neutrophil depletion with Ly6G antibody
- Peripheral blood neutrophil count
- WT (wild-type) mice
- IP6K1-deficient mice
- Positive control: WT (wild-type) mice
- Negative control: IP6K1-deficient mice
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!