Methanobrevibacter arboriphilus strain DH1 (DSM 1125) was obtained from the Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ), Braunschweig, Germany. The methanogenic archaeon was cultivated under anaerobic conditions using a medium described by Asakawa et al. [12 ]. DNA was isolated from the enrichment culture by phenol-chloroform extraction followed by a QIAquick column (Qiagen, Hilden, Germany) purification [23 (link), 24 (link)].
Cultivation of Methanobrevibacter arboriphilus
Methanobrevibacter arboriphilus strain DH1 (DSM 1125) was obtained from the Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ), Braunschweig, Germany. The methanogenic archaeon was cultivated under anaerobic conditions using a medium described by Asakawa et al. [12 ]. DNA was isolated from the enrichment culture by phenol-chloroform extraction followed by a QIAquick column (Qiagen, Hilden, Germany) purification [23 (link), 24 (link)].
Corresponding Organization : National University of Singapore
Other organizations : University of Göttingen
Variable analysis
- Methanobrevibacter arboriphilus strain DH1 (DSM 1125) cultivation conditions
- DNA isolation from the enrichment culture
- Not explicitly mentioned
- No positive or negative controls were explicitly mentioned in the provided information.
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