Methanobrevibacter arboriphilus strain DH1 (DSM 1125) was obtained from the Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ), Braunschweig, Germany. The methanogenic archaeon was cultivated under anaerobic conditions using a medium described by Asakawa et al. [12 ]. DNA was isolated from the enrichment culture by phenol-chloroform extraction followed by a QIAquick column (Qiagen, Hilden, Germany) purification [23 (link), 24 (link)].
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