Bumetanide Dosage for Neonatal Seizures

The effective systemic dose of Bumetanide on neonatal seizures in rats is 0.1–0.5 mg/kg (21 (link), 25 (link)). We administered 0.2 mg/kg of Bumetanide twice a day in repeated doses as previously described (26 (link)). Bumetanide (B3023; Sigma-Aldrich) was dissolved in 100% ethyl alcohol and then diluted with 0.9% saline to a final concentration of 0.05 mg/mL. Bumetanide solution (0.2 mg/kg) or equal volumes of 0.9% saline (4 mL/kg) was injected twice daily from P12 to P20 or P26. MEMRI was performed on P21 or P27 for each rat.

Free full text: Click here

Tahara M., Higurashi N., Hata J., Nishikawa M., Ito K., Hirose S., Kaneko T., Mashimo T., Sakuma T., Yamamoto T, & Okano H.J. (2023). Developmental changes in brain activity of heterozygous Scn1a knockout rats. Frontiers in Neurology, 14, 1125089.

Publication 2023

Bumetanide

0 9 saline Bumetanide Ethyl alcohol Neonatal Seizures

Corresponding Organization : Jikei University School of Medicine

Other organizations : Tokyo Metropolitan University, Fukuoka University, Iwate University, The University of Tokyo, Tokyo University of Science, Hiroshima University

Top 5 similar protocols

Variable analysis

independent variables

Dose of bumetanide (0.2 mg/kg)
Frequency of administration (twice a day)
Duration of administration (from P12 to P20 or P26)

dependent variables

Neonatal seizures in rats

control variables

Administration of 0.9% saline (4 mL/kg) twice daily
Measurement of MEMRI on P21 or P27 for each rat

controls

Positive control: Not explicitly mentioned
Negative control: Administration of 0.9% saline (4 mL/kg) twice daily

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!