Transcriptome Analysis of Mycobacterium tuberculosis

M. tb strains were cultured as described above with shaking at 220 rpm till A₅₉₅ ~ 0.2–0.3. A 50 ml aliquot was centrifuged and RNA was isolated using TRI reagent method as described [16 (link)]. Reverse transcription was performed on the total extracted RNA with random hexamer primers and cDNA High capacity Reverse Transcriptase kit (ABI, USA). mRNAs were quantitated by real-time PCR using gene-specific primers (Additional file 1: Table S1) in MyIQ thermal cycler (Biorad, USA). For whole genome transcriptome, the data deposited with NCBI GEO datasets (Accession number GSE30264) was analysed as described previously [16 (link)].