Inducible CEP120-GFP in RPE1 cells

The U2OS-based doxycycline inducible PLK4-myc cell line used in this study was as previously described^{40 (link)}. To obtain CEP120-null RPE1 cell lines inducibly expressing CEP120-GFP (WT or mutants), lentiviruses containing CEP120-GFP (WT or mutants) in the pLVX-tight-puro vector (BD Biosciences Clontech) were used to infect CEP120-null RPE1 Tet-On cells that stably express rtTA. The infected cells were selected with 10 μg/ml puromycin or were sterile-sorted by cell sorter (FACSAria, BD Biosciences) for GFP signal. The positive cells were selected and expanded as inducible lines. The expression of CEP120-GFP (WT or mutants) was induced by adding 1 μg/ml doxycycline to the culture medium.