Assessing Cancer Cell Migration

Cancer cell migration was assessed by using the Oris^TM Cell migration kit (Platypus Technology, Madison, WI), as previously described [38 (link)]. MDA-MB-231- fibroblast or macrophage conditioned media (100 μl) with or without 0.1 uM reparixin (MCE, Monmouth Junction, NJ) and anti-IL-8 antibodies (R&D Systems, 30 μg/ml) was added once the cancer cells had attached. Migration and proliferation assays using CIM-plates (Roche, Indianapolis, IN) and the RTCA system (ACEA Bioscience, San Diego, CA) were performed as previously described [47 (link)].

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Jin K., Pandey N.B, & Popel A.S. (2017). Crosstalk between stromal components and tumor cells of TNBC via secreted factors enhances tumor growth and metastasis. Oncotarget, 8(36), 60210-60222.

Publication 2017

anti-IL-8 antibodies CIM-plates RTCA system

Anti antibodies Assays Cancer Cell migration Cells Conditioned media Fibroblast Macrophage Platypus Reparixin Rtca

Corresponding Organization :

Other organizations : Johns Hopkins Medicine, Johns Hopkins University, Sidney Kimmel Comprehensive Cancer Center

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Variable analysis

independent variables

Reparixin (0.1 uM)
Anti-IL-8 antibodies (30 μg/ml)

dependent variables

Cancer cell migration
Cancer cell proliferation

control variables

MDA-MB-231-fibroblast or macrophage conditioned media

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