Immunoblotting of ER Stress Markers

Primary antibodies for SERCA (1:200, #271669; Santa Cruz Biotechnology, CA, USA), C/-EBP homologous protein (CHOP, 1:100, Santa Cruz Biotechnology, #7351), Bcl-2 (1:500, # 4223S; Cell Signaling Technology, Danvers, MA, USA), p-PERK (1:500, #156919; Abcam, Cambridge, U.K.), caspase-3 (1:200, #56053; Santa Cruz Biotechnology), and β-actin (1:2000, #47778; Santa Cruz Biotechnology) were purchased and maintained overnight at 4 °C. The detailed protocol can be found in our previous study [27 (link)].

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Chang H.S., Kim Y., Lee S.Y., Yun H.J., Chang H.J, & Park K.C. (2023). Anti-Cancer SERCA Inhibitors Targeting Sorafenib-Resistant Human Papillary Thyroid Carcinoma. International Journal of Molecular Sciences, 24(8), 7069.

Publication 2023

C/-EBP homologous protein (CHOP Bcl-2 p-PERK caspase-3 β-actin

Actin Antibodies Bcl 2 Caspase 3 Chop

Corresponding Organization : Yonsei University

Other organizations : Genome and Company (South Korea)

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Variable analysis

independent variables

Primary antibodies for SERCA
Primary antibodies for C/-EBP homologous protein (CHOP)
Primary antibodies for Bcl-2
Primary antibodies for p-PERK
Primary antibodies for caspase-3
Primary antibodies for β-actin

dependent variables

Not explicitly mentioned

control variables

Primary antibody concentrations (1:200, 1:100, 1:500, 1:500, 1:200, 1:2000)
Primary antibody incubation temperature (4 °C)
Primary antibody incubation time (overnight)

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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