Conditional Transgenic Mice for IDH1/2 Mutations

The conditional Jak2^V617F mice were previously described (13 (link)). Conditional transgenic IDH1^R132H mice (expressing a mutation in the IDH1 gene) were provided by Kwok-Kin Wong (Dana-Farber Cancer Institute), and conditional Idh2^R140Q mice were provided by Craig B. Thompson (Memorial Sloan Kettering Cancer Center). All mice were maintained on a C57BL/6J background, and the lines were backcrossed for 6 generations before use in experiments. Samples for experiments were obtained from male and female mice aged 6 months to 1.5 years. Upon induction, mice received 5 i.p. injections of polyI:polyC (Amersham) of 200 μl of a 1-mg/ml solution. Peripheral blood was collected via cheek bleeding using heparinized microhematocrit capillary tubes (Thermo Fisher Scientific). Peripheral blood counts were obtained using a HemaVet according to the manufacturer’s instructions. Excision 2 weeks after induction was confirmed using PCR.