Fatty Acid Profiling by GC

Fatty acid profiles of mouse diets and tail tissues (n = 9–10 per group) were analyzed by GC as described previously^{16 (link),55 (link)}. Briefly, tissue or food samples were ground to powder under liquid nitrogen and subjected to total lipid extraction and fatty acid methylation by 14% boron trifluoride (BF3)-methanol reagent (Sigma-Aldrich) at 100°C for 1 h. Fatty acid methyl esters were analyzed using a fully automated HP5890 GC system equipped with a flame-ionization detector (Agilent Technologies, Palo Alto, CA). The fatty acid peaks were identified by comparing their relative retention times with the commercial mixed standards (NuChek Prep, Elysian, MN), and area percentage for all resolved peaks was analyzed by using a Perkin-Elmer M1 integrator.

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Kaliannan K., Li X.Y., Wang B., Pan Q., Chen C.Y., Hao L., Xie S, & Kang J.X. (2019). Multi-omic analysis in transgenic mice implicates omega-6/omega-3 fatty acid imbalance as a risk factor for chronic disease. Communications Biology, 2, 276.

Publication 2019

HP5890 GC system flame-ionization detector M1 integrator

Boron trifluoride Diets Esters Fatty acid Flame ionization Food Lipid Methanol Methylation Mouse Nitrogen Powder Retention Tail Tissue

Corresponding Organization :

Other organizations : Massachusetts General Hospital, Harvard University

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Variable analysis

independent variables

Mouse diets

dependent variables

Fatty acid profiles of mouse tail tissues

control variables

Sample preparation (ground to powder under liquid nitrogen, total lipid extraction, and fatty acid methylation)
Analytical method (GC with flame-ionization detector)
Fatty acid peak identification (by comparing relative retention times with commercial standards)
Data analysis (area percentage for all resolved peaks)

controls

Positive control: Commercial mixed standards (NuChek Prep, Elysian, MN)
Negative control: Not explicitly mentioned

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