To visualize and assess the area of hM3D(Gq)-mCherry transduced neurons, the laser wavelength was changed to 990 nm for overview imaging. Ca2+ recordings were performed at a wavelength of 940 nm. hM3D(Gq) DREADD transduced neurons were activated with 30 µg/kg bodyweight clozapine (Tocris, Cat. No. 0444) (Jendryka et al., 2019 (link)) in saline (0.9% w/v) via a tail vein injection during image acquisition.
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