Lung, brain, heart, and kidney tissues taken from the subjects were fixed in 10% neutral formalin. Sample tissues were submerged in paraffin after being put through degreed alcohol and xylene. 5-μm-thick sections were taken and dyed with hematoxylin eosin and toluidine blue for general examination and for obtaining the mast cell count, respectively; and they were assessed using a BH2 Olympus light microscope. Histopathological evaluations were performed on each subject’s lung, brain, heart, and kidney sample sections. Changes in the lung tissue were scored in terms of alveolar edema, hemorrhage, cell infiltration, and alveolar wall thickness. Scoring was assigned as normal structure (0), minor changes (1), moderate changes (2), and widespread changes (3) [15 (link)]. In the lung tissue of all subjects, the mast cell count was obtained at 40× scope magnification in 6 different areas. Histopathological scoring was performed by a researcher who was not informed about the subject groups.
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