Comprehensive Western Blotting Protocol

Western blotting was performed as described previously [22 (link)]. SDS-PAGE electrophoresis, transfer to nitrocellulose membrane and immunoblotting with ECL (Thermo Scientific, USA) detection were performed according to standard manufacturer’s protocols (Bio-Rad Laboratories, USA). Antibodies against the following proteins were used: glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (clone 14C10) (1:1000, #2118, Cell Signaling, USA), phospho-p53 (Ser15) (clone 16G8) (1:700, #9286, Cell Signaling, USA), p21Waf1/Cip1 (clone 12D1) (1:1000, #2947, Cell Signaling, USA), phospho-Rb (Ser807/811) (1:1000, #8516, Cell Signaling, USA), phospho-ATM (Ser1981) (clone D6H9) (1:1000, #5883, Cell Signaling, MA, USA), phospho-Histone H2A.X (Ser139) (clone JBW301) (1:1000, #05-636, Merck Millipore, Germany), CD63 (1:500, ab68418, Abcam, UK), HSP70 (clone 2H9) (#MABE1130, Merck Millipore, Germany), PAI-1 (D9C4) (1:1000, #11907, Cell Signaling, USA) as well as horseradish peroxidase-conjugated goat anti-rabbit IG (GAR-HRP, Cell Signaling, USA) (1:10000) and antimouse IG (GAM-HRP, Cell Signaling, USA) (1:10000). Full size blots are provided in the Supplementary Figure 1.