Evaluation of Small Cell Lung Cancer Cell Line Growth Inhibition

All the SCLC cell lines were purchased from American Type Culture Collection (Manassas, VA) and cultured as recommended. All these cell lines were passaged fewer than 6 months after purchase. MCL1 knockout (MCL1^−/−) MEF cells were kindly provided by Dr. David Huang (The Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia). Cell growth inhibition was evaluated by means of a WST-8 assay (Dojindo, Rockville, MD) as described previously [24] (link). Mitochondrial membrane potential was determined by JC-1 staining using a JC-1 Mitochondrial Membrane Potential Assay kit (Cayman). Apoptosis analysis was performed using an Annexin V/propidium iodide (PI) apoptosis detection kit (Roche Applied Sciences, Indianapolis, IN).