LIG electrodes were fabricated on a Kapton film substrate using a CO2 laser (VLS2.30DT, Universal Laser Systems, Inc., Scottsdale, AZ, US) at 75% speed, 40% power, and 1000 PPI (Lin et al., 2014 (link)). The working electrode was designed in CorelDraw with a circular working area (ϕ = 3.0 mm), connected to a stem (14.3 × 2.0 mm), that leads to a rectangular bonding pad (2.9 × 2.5 mm) (Supplementary Figure S2 ). A nitrocellulose passivation layer was applied on the stem area, and a metallic tape was incorporated on the bonding pad area for enhancing shear strength. The LIG electrodes were further modified with platinum nanoparticles (nPt) via electrodeposition by connecting the LIG electrode to the anode and a platinum wire to the cathode of a DC power supply (HM305P, HANMATEK). Next, LIG and Pt wire were immersed in a solution of 1.44% (v/v) chloroplatinic acid and 0.002% (v/v) lead acetate. The DC power supply was programmed to hold a constant potential of 10 V for 90s during the electroplating process.
Fabricated LIG-nPt electrodes were tested via electrochemical methods using a benchtop MultiPalmSens4 potentiostat (PalmSens, Houten, Netherlands) connected to a 3-electrode cell stand. The three-electrode system consisted of an Ag/AgCl (3M KCl) reference electrode (BASi®, West Lafayette, IN, United States), platinum wire auxiliary electrode (BASi®, West Lafayette, IN, United States), and LIG-nPt working electrode. Cyclic voltammetry was carried out in a solution containing KCl (100 mM), K3 [Fe(CN)6] (2.5 mM), and K4 [Fe(CN)6] (2.5 mM) using a potential range from −0.8 to 0.8 V at a scan rate of 200 mV/s for 10 cycles. The resulting voltammograms were used to select replicate LIG-nPt electrodes for further biofunctionalization and testing.
Fabricated LIG-nPt electrodes were tested via electrochemical methods using a benchtop MultiPalmSens4 potentiostat (PalmSens, Houten, Netherlands) connected to a 3-electrode cell stand. The three-electrode system consisted of an Ag/AgCl (3M KCl) reference electrode (BASi®, West Lafayette, IN, United States), platinum wire auxiliary electrode (BASi®, West Lafayette, IN, United States), and LIG-nPt working electrode. Cyclic voltammetry was carried out in a solution containing KCl (100 mM), K3 [Fe(CN)6] (2.5 mM), and K4 [Fe(CN)6] (2.5 mM) using a potential range from −0.8 to 0.8 V at a scan rate of 200 mV/s for 10 cycles. The resulting voltammograms were used to select replicate LIG-nPt electrodes for further biofunctionalization and testing.
Free full text: Click here
