Genomic DNA Extraction and Sequencing Protocol

Genomic DNA was extracted from colonies grown on cellophane-covered PDA using a modified CTAB method (Doyle and Doyle 1990 (link)). The primers and PCR conditions are listed in Table 2. DNA sequencing was performed using an ABI PRISM 3730XL DNA Analyser with a BigDye Terminator Kit v.3.1 (Invitrogen, USA) at the Shanghai Invitrogen Biological Technology Company Limited (Beijing, China). The DNA sequences obtained from forward and reverse primers were combined using SeqMan v. 7.1.0 in the DNASTAR Lasergene Core Suite software (DNASTAR Inc., Madison, WI, USA). Reference sequences were selected based on ex-type or ex-epitype sequences available from relevant recently published literature (Rossman et al. 2007 (link), Suetrong et al. 2015 (link), Norphanphoun et al. 2016 (link), Hongsanan et al. 2017 (link), Senanayake et al. 2017 (link), Voglmayr et al. 2017 (link), Yang et al. 2018 (link), Fan et al. 2018a (link), b (link), 2020 (link)) (Table 1). Subsequent alignments for each gene were generated using MAFFT v.7 (Katoh and Standley 2013 (link)) and manually improved where necessary using MEGA v. 6 (Tamura et al. 2013 (link)). Novel sequences generated in the current study were deposited in GenBank (Table 1, Suppl. materials 1–3: Tables S1–S3) and the aligned matrices used for phylogenetic analyses were submitted to TreeBASE (www.treebase.org; accession number: S24893).