Western blotting was performed as described previously (21 (link)). The following antibodies were used: Rabbit antibodies against HNF-4α (cat. no. ab201460; Abcam), E-cadherin (cat. no. 24E10; Cell Signaling Technology, Inc.), N-cadherin (cat. no. 13116; Cell Signaling Technology, Inc.), β-catenin (cat. no. 8480; Cell Signaling Technology, Inc.), phosphorylated (P)-p65 (cat. no. 3033; Cell Signaling Technology, Inc.), p65 (cat. no. 8242; Cell Signaling Technology, Inc.), P-STAT3 (cat. no. ab76315; Abcam), P-AKT (cat. no. ab38449; Abcam), AKT (cat. no. ab8805; Abcam) and β-actin monoclonal antibody (cat. no. HRP-60008; Proteintech Group, Inc.); mouse antibodies against STAT3 (cat. no. ab119352; Abcam).
Protocol detail
Comprehensive Western Blotting Protocol for Cellular Signaling Analysis
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Corresponding Organization :
Other organizations : Shanghai Tenth People's Hospital, Tongji University
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Variable analysis
independent variables
- Western blotting was performed as described previously (21 (link)). The following antibodies were used: Rabbit antibodies against HNF-4α, E-cadherin, N-cadherin, β-catenin, phosphorylated (P)-p65, p65, P-STAT3, P-AKT, AKT and mouse antibodies against STAT3.
- Not explicitly mentioned.
- Not explicitly mentioned.
- No positive or negative controls were specified by the authors.
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