Differentiation of hiPSCs into Pancreatic Islets

The information on hiPSC and human islets was displayed in Supplementary Tables S1, S2. Human primary islets were maintained in CMRL 1066 (Corning, 15-110-CV) supplemented with 5% human AB serum (PAN-Niotech GmbH), L-Glutamine, 1% penicillin/streptomycin, 10 mM HEPES (all from Gibco) on ultra-low attachment plates (Corning, CLS3261). The hiPSCs were cultured in E8 Medium (Gibco, A1517001), and confirmed to be mycoplasma-free. The differentiation was done using the seven stages protocol [3 (link)] with modification [12 (link)]. On day 1 of the suspension culture, Rho Kinase inhibitor Y27632 (StemCell Technologies, 72304) was added to prevent cell death (Supplementary Table S3).