Generation of PHA-activated T cell blasts

Peripheral blood mononuclear cells (PBMCs) were obtained from healthy volunteers with informed consent using a Baylor College of Medicine institutional review board-approved protocol (H-45017) and were used to generate phytohemagglutinin (PHA) blasts and multiVSTs. PHA blasts were generated as previously reported (9 (link)) and cultured in T cell medium [45% RPMI 1640 (HyClone Laboratories, Logan, Utah), 45% Click’s medium (Irvine Scientific, Santa Ana, California), 2 mM GlutaMAX TM-I (Life Technologies, Grand Island, New York), and 5% human AB serum (Valley Biomedical, Winchester, Virginia)] supplemented with 100U/ml interleukin 2 [IL2; Proleukin^® (aldesleukin), TCH, Houston, TX], which was replenished every 2 days.

Free full text: Click here

Koukoulias K., Papayanni P.G., Jones J., Kuvalekar M., Watanabe A., Velazquez Y., Gilmore S., Papadopoulou A., Leen A.M, & Vasileiou S. (2023). Assessment of the cytolytic potential of a multivirus-targeted T cell therapy using a vital dye-based, flow cytometric assay. Frontiers in Immunology, 14, 1299512.

Publication 2023

RPMI 1640 Click’s medium GlutaMAX TM-I human AB serum

Corresponding Organization : Houston Methodist

Other organizations : Allure (United States), G. Papanikolaou General Hospital

Top 5 similar protocols

Variable analysis

independent variables

None explicitly mentioned

dependent variables

None explicitly mentioned

control variables

Peripheral blood mononuclear cells (PBMCs) were obtained from healthy volunteers
PHA blasts were generated as previously reported
PHA blasts were cultured in T cell medium supplemented with 100U/ml interleukin 2 (IL2), which was replenished every 2 days

controls

Positive control: Not specified
Negative control: Not specified

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!