Transient Expression of Potato Proteins

The CDS of StTCP15 was cloned into the KpnI and XbaI sites of the pCAMBIA1300-cLUC vector, and the StSnRK1, StF-box, and StGID1 without terminator CDS were ligated to the SacI and SalI sites of the pCAMBIA1300-nLUC vector. Specific primers were shown in Supplementary Table 1. The recombinant plasmid was transformed into Agrobacterium tumefaciens strain GV3101. As described by Chen et al., pCAMBIA1300-cLUC-StTCP15 and pCAMBIA1300-nLUC-StSnRK1/StF-box/StGID1 were mixed in equal volumes, and the injection and infection methods were the same as the subcellular localization methods. pCAMBIA1300-cLUC and pCAMBIA1330-nLUC served as blank controls. Nicotiana benthamiana leaves were sprayed with D-luciferin potassium salt (Solarbio, Beijing, China), and the fluorescence was detected by a plant live imaging system (BLT PlantView100, Guangzhou Biolight Biotechnology Co., Ltd., Guangzhou, China; Chen et al., 2022 (link)).