Generating Transgenic Mice for Imaging

C57BL/6, 7 weeks old, male CX3CR1^GFP/GFP, and CCR2^RFP/RFP mice were obtained from Jackson Laboratory. The generative protocols for the CX3CR1^GFP/GFP and CCR2^RFP/RFP mice were described previously [14 (link), 26 (link)]. We generated C57BL/6, 12 weeks old, male CX3CR1^GFP/+, CCR2^RFP/+, and CX3CR1^GFP/+-CCR2^RFP/+ dual-reporter transgenic mice by mating the CX3CR1^GFP/GFP and CCR2^RFP/RFP mice with C57BL/6 wild type mice or each other. All animal experiments were approved by the Institutional Animal Care and Use Committee (IACUC) at Yonsei Laboratory Animal Research Center (YLARC). Animals were housed with food and water ad libitum and kept on a 12-h light/dark cycle.