Co-Immunoprecipitation Assay for Protein Interactions

Co-immunoprecipitation (co-IP) was performed as previously described [14 (link)–16 (link)], with antibodies for p113 (ABclonal Biotechnology Co., Ltd), GST-tag (sc-33614, Santa Cruz Biotechnology), Flag-tag (14793S), ZRF1 (12844S), BRD4 (13440S), HA-tag (3724S), His-tag (12698S), MBP-tag (2396S, Cell Signaling Technology Inc., MA). Bead-bound proteins were analyzed by Coomassie blue staining, western blot, or mass spectrometry (Wuhan Institute of Biotechnology, Wuhan, China).

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Yang F., Hu A., Guo Y., Wang J., Li D., Wang X., Jin S., Yuan B., Cai S., Zhou Y., Li Q., Chen G., Gao H., Zheng L, & Tong Q. (2021). p113 isoform encoded by CUX1 circular RNA drives tumor progression via facilitating ZRF1/BRD4 transactivation. Molecular Cancer, 20, 123.

Publication 2021

Flag-tag HA-tag (3724S His-tag (12698S

Antibodies Brd4 Co immunoprecipitation Coomassie blue Mass spectrometry P113 Proteins Western blot

Corresponding Organization :

Other organizations : Union Hospital, Huazhong University of Science and Technology

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Variable analysis

independent variables

Antibodies for p113 (ABclonal Biotechnology Co., Ltd)
GST-tag (sc-33614, Santa Cruz Biotechnology)
Flag-tag (14793S)
ZRF1 (12844S)
BRD4 (13440S)
HA-tag (3724S)
His-tag (12698S)
MBP-tag (2396S, Cell Signaling Technology Inc., MA)

dependent variables

Bead-bound proteins analyzed by Coomassie blue staining, western blot, or mass spectrometry (Wuhan Institute of Biotechnology, Wuhan, China)

control variables

Co-immunoprecipitation (co-IP) procedure previously described [14 (link)–16 (link)]

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