Transmission Electron Microscopy of Leaf Samples

The Gy14 and yf leaf samples were collected from the 14th true leaves for TEM. Leaf samples of the 9930 WT and CsSRP43 CRISPR transgenic lines were collected from the second expanded leaf. These leaves were cut into small pieces of ~0.2 mm × 0.2 mm size and fixed in a 4.0% glutaraldehyde solution. Leaf samples were prepared for TEM according to Xiong et al. [67 (link)]. Finally, the samples were observed using a Tecnai G2 Spirit Bio (FEI, USA) transmission electron microscope.

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Zhang T., Dong X., Yuan X., Hong Y., Zhang L., Zhang X, & Chen S. (2022). Identification and characterization of CsSRP43, a major gene controlling leaf yellowing in cucumber. Horticulture Research, 9, uhac212.

Publication 2022

Tecnai G2 Spirit Bio

Crispr Glutaraldehyde Leaf Transgenic Transmission electron microscope

Corresponding Organization : Northwest A&F University

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Variable analysis

independent variables

Gy14 leaf samples
yf leaf samples
9930 WT leaf samples
CsSRP43 CRISPR transgenic leaf samples

dependent variables

Characteristics of leaf samples observed using transmission electron microscope (TEM)

control variables

Leaf samples were collected from the 14th true leaves for Gy14 and yf samples, and from the second expanded leaf for 9930 WT and CsSRP43 CRISPR transgenic samples
Leaf samples were cut into small pieces of ~0.2 mm × 0.2 mm size
Leaf samples were fixed in a 4.0% glutaraldehyde solution
Leaf samples were prepared for TEM according to Xiong et al. [67 (link)]
Samples were observed using a Tecnai G2 Spirit Bio (FEI, USA) transmission electron microscope

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