The 3-week-old C57BL/6 male mice were randomly divided into 4 groups: control group (n = 22, routine dietary water with intraperitoneal injection of isotonic sodium chloride solution), Cipro group (n = 23, routine dietary water with intraperitoneal injection of ciprofloxacin), BAPN (Aladdin) (1 mg/g/d) group (n = 48, BAPN dissolved in drinking water with intraperitoneal injection of isotonic sodium chloride solution), and BAPN+Cipro group (n = 49, BAPN dissolved in drinking water with intraperitoneal injection of ciprofloxacin). We selected the concentration of ciprofloxacin according to the standard low dose (8.3 mg/kg/d) for the treatment of patients with common indications and converted it to the equivalent dose concentration in mice (40 mg/kg/d, intraperitoneal injection) (48 (link)–50 (link)). In all animal experiments, BAPN diluted in daily drinking water was used for 28 days. Ciprofloxacin or isotonic sodium chloride solution was injected intraperitoneally from weeks 2 to 6. AA/AD was defined as previously described (12 (link)). At the end of the study, the mice were sacrificed, and the aortas were processed for AA/AD evaluation and tissue analysis.
We validated the hypothesis that ciprofloxacin could induce apoptosis through MAPK-dependent activation of Bax/Bcl2 signaling in vivo. Three-week-old C57BL/6 male mice were randomly divided into 3 groups: the BAPN group (n = 35, BAPN dissolved in drinking water with intraperitoneal injection of isotonic sodium chloride solution), BAPN+Cipro group (n = 35, BAPN dissolved in drinking water with intraperitoneal injection of ciprofloxacin), and BAPN+Cipro+MAPK inhibitor group (n = 36, BAPN dissolved in drinking water with intraperitoneal injection of ciprofloxacin and SB203580 [0.5 mg/kg/d] and U0126 [10 mg/kg/d]). In all animal experiments, BAPN was administered for 28 days. Ciprofloxacin or isotonic sodium chloride solution was injected intraperitoneally from weeks 2 to 6, while inhibitors were administered from weeks 1 to 6.
We validated the hypothesis that ciprofloxacin could induce apoptosis through MAPK-dependent activation of Bax/Bcl2 signaling in vivo. Three-week-old C57BL/6 male mice were randomly divided into 3 groups: the BAPN group (n = 35, BAPN dissolved in drinking water with intraperitoneal injection of isotonic sodium chloride solution), BAPN+Cipro group (n = 35, BAPN dissolved in drinking water with intraperitoneal injection of ciprofloxacin), and BAPN+Cipro+MAPK inhibitor group (n = 36, BAPN dissolved in drinking water with intraperitoneal injection of ciprofloxacin and SB203580 [0.5 mg/kg/d] and U0126 [10 mg/kg/d]). In all animal experiments, BAPN was administered for 28 days. Ciprofloxacin or isotonic sodium chloride solution was injected intraperitoneally from weeks 2 to 6, while inhibitors were administered from weeks 1 to 6.
Free full text: Click here
