Bovine Zoonosis Surveillance in Spain

The reference population consisted of 75 Spanish Holstein cows from a single farm located in the Basque Country. In 2022, only five cows from this herd had an ELISA positive result and all the animals had a negative fecal PCR result. Only adult cows (2 years or older, 4.5 years mean age) were included in the study. All the animals included in this study were registered with the Spanish Federation of Holstein cattle (CONAFE; www.conafe.com.) and had negative fecal PCR results when blood samples were collected and in subsequent annual samplings. In addition, the animals used in this study were not diagnosed with any other pathogen. Blood samples were collected in groups of 16 per sampling day. The validation population consisted of 16 cows from the same farm.

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Badia-Bringué G., Canive M, & Alonso-Hearn M. (2023). Control of Mycobacterium avium subsp. paratuberculosis load within infected bovine monocyte-derived macrophages is associated with host genetics. Frontiers in Immunology, 14, 1042638.

Publication 2023

Adult Animals Blood Cows Elisa Fecal Holstein cows Pathogen Spanish

Corresponding Organization : Euskadiko Parke Teknologikoa

Other organizations : University of the Basque Country

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

ELISA positive result
Fecal PCR result

control variables

Spanish Holstein cows from a single farm located in the Basque Country
Only adult cows (2 years or older, 4.5 years mean age)
All animals registered with the Spanish Federation of Holstein cattle (CONAFE)
Blood samples collected in groups of 16 per sampling day
Validation population consisted of 16 cows from the same farm

controls

Positive control: None mentioned
Negative control: All animals had a negative fecal PCR result

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