Apoptosis Assay in OS Cells

OS cells were seeded in 6-cm plates and incubated with different concentrations of AIL. After 24 h, all the cells including the floating and adherent ones were harvested and stained with Annexin V-fluorescein isothiocyanate (FITC) and propidium iodide (PI) apoptosis detection kit (#556547, BD Biosciences Pharmingen, San Diego, CA, USA) according to the manufacturer’s protocols. The resulting fluorescence was assessed by fluorescence-activated cell sorting scan (FACS) flow cytometry (Becton Dickinson, Mountain View, CA, USA) as reported previously (25 (link)).

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Zhang Y., Gong R., Liu Y., Sun X., Liang J., Zhou Y., Wang Y., Yu W., Wang Y., Tang L., He A., Shen Z., Yao Y., Hu H., Liu X, & Zhang J. (2022). Ailanthone Inhibits Proliferation, Migration and Invasion of Osteosarcoma Cells by Downregulating the Serine Biosynthetic Pathway. Frontiers in Oncology, 12, 842406.

Publication 2022

Annexin V-fluorescein FACS) flow cytometry

Annexin v Apoptosis Cells Flow cytometry Fluorescence Isothiocyanate Propidium iodide Scan

Corresponding Organization : Fudan University Shanghai Cancer Center

Other organizations : Guangxi University for Nationalities, Shanghai Eighth People Hospital

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Variable analysis

independent variables

Different concentrations of AIL

dependent variables

Apoptosis (measured by Annexin V-FITC and propidium iodide staining)

control variables

OS cells
6-cm plates
Incubation time (24 h)
Annexin V-FITC and propidium iodide apoptosis detection kit (#556547, BD Biosciences Pharmingen, San Diego, CA, USA)
Fluorescence-activated cell sorting scan (FACS) flow cytometry (Becton Dickinson, Mountain View, CA, USA)

controls

No positive or negative controls were explicitly mentioned in the provided information.

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