Ultrastructural Analysis of Kidney after GOs Treatment

The ultrastructural changes of the kidney after GOs treatment were observed by TEM (JEOL JEM-1400) imaging. The kidney tissues were prefixed with 2.5% glutaraldehyde at 4 °C overnight and then post-fixed in 1% osmium tetroxide at 4 °C for 3 h. The samples were sectioned to 70 nm thick after dehydration and resin embedding, stained with uranyl acetate and lead citrate. The glomerular ultrastructural changes, including the glomerular basement membrane (GBM) thickness, podocyte foot process width and length, and glomerular slit diaphragm perimeter were quantitatively analyzed using ImageJ 1.51 k software.

Free full text: Click here

Chen W., Wang B., Liang S., Wang M., Zheng L., Xu S., Wang J., Fang H., Yang P, & Feng W. (2023). Renal clearance of graphene oxide: glomerular filtration or tubular secretion and selective kidney injury association with its lateral dimension. Journal of Nanobiotechnology, 21, 51.

Publication 2023

JEM-1400

Citrate Dehydration Diaphragm Foot Glomerular Glomerular basement membrane Glutaraldehyde Kidney Osmium tetroxide Perimeter Podocyte Resin Tissues Uranyl acetate

Corresponding Organization :

Other organizations : Institute of High Energy Physics, Chinese Academy of Sciences, University of Chinese Academy of Sciences

Top 5 similar protocols

Variable analysis

independent variables

Treatment with GOs (Graphene Oxide)

dependent variables

Glomerular ultrastructural changes, including:
- Glomerular basement membrane (GBM) thickness
- Podocyte foot process width and length
- Glomerular slit diaphragm perimeter

control variables

Kidney tissue fixation with 2.5% glutaraldehyde at 4 °C overnight
Post-fixation in 1% osmium tetroxide at 4 °C for 3 h
Sectioning to 70 nm thick after dehydration and resin embedding
Staining with uranyl acetate and lead citrate

controls

No positive or negative controls were explicitly mentioned in the provided information.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!