Phenotypic Characterization of brDFAT and wDFAT Cells

For phenotypic characterization of the brDFAT and wDFAT cells, the cells were cultured in culture medium until 100% confluent. Fluorescence-activated cell sorting (FACS) analysis was performed after the first passage as previously described^{14 (link)} using the following conjugated anti-mouse antibodies: Sca1 (Ly-6A/E) [Phycoerythrin (PE), BD Biosciences, Cat. No. 553336), c-Kit (CD117) [Fluorescein isothiocyanate (FITC), BD Biosciences, Cat. No. 553354], CD105 (PE/Cyanine7, BioLegend, Cat. No. 120409), CD11b (Alexa Fluor 488, BioLegend, Cat. No. 101219), CD90 (PE, eBioscience, Cat. No. 12–0900-81), SSEA-1 (CD15) (Alexa Fluor 488, BioLegend, Cat. No. 125609), CD31 (PECAM1) (PE, BD Biosciences, Cat. No. 553373) and CD34 (FITC, eBioscience, Cat. No. 11–0341-85), all diluted 1:100 in 1% BSA.