Myocardial Infarction and Cell Therapy in Rats
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Corresponding Organization : Rice University
Variable analysis
- Presence of myocardial infarction (MI) induced by ligation of the left anterior descending (LAD) coronary artery
- Delivery method of mesenchymal stem cells (MSCs): either as cell capsules deposited on the epicardial surface or as direct intramyocardial injection
- Visual confirmation of infarction by edema and discoloration of the anterior LV wall
- Anesthesia with inhalational isoflurane in 100% O2 (5.0% induction; 2.5% maintenance)
- Endotracheal intubation and positive-pressure ventilation
- Left lateral thoracotomy procedure
- Pericardial sac identification and opening to expose the left ventricle
- Deposition of blank cell capsules on the epicardial surface of the right and left ventricle for control animals
- Positive control: Animals that underwent MI induction by LAD ligation
- Negative control: Animals that received blank cell capsules (no MSCs)
Annotations
Based on most similar protocols
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!