We used breast cancer (HCC1569, HCC1954, BT549, MDA-MB-415, HCC202, MCF7, T47D, CAL51), ovarian cancer (KOC-7C, SKOV3, HEYC2, COLO704, RMG-1, KK, ES-2, EFO21, MCAS, A2780, OV167, PEA2, TOV-21G, OV90, OV207, Kuramochi, OVISE, OVMANA, PEO6, CaOv-3, OV177, OVCAR3, OVCAR5, OVSAHO, OVTOKO, PEO14), colorectal cancer (HCT-116, HCT-15, DLD-1, COLO 205, COLO320DM, COLO320HSR, NCI-H747, NCI-H716, COLO201, LS1034, SNU-C2B, SNU-C1, LS513, LS411N, ATR-FLOX, LS174t, WiDr, RKO, SK-CO1, HCT-8, LS-180, HT-29, C2Bbe1, SW480), and immortalized breast epithelial (MCF-10–2A, MCF-12A) cell lines from the American Type Culture Collection (ATCC) or the Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ) for our experiments grown as described elsewhere (42 (link)–44 (link, link)). Cultured cells were synchronized as needed by using single or double thymidine blockade. For experiments with CFI-400945, the PLK4 inhibitor was added to medium at 10 nM, 50 nM, 100 nM, or 500 nM concentrations and compared with results with DMSO controls.
Protocol detail
Inhibition of PLK4 in Cancer Cell Lines
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Breast
Breast cancer
Cfi 400945
Colorectal cancer
Culture cell
Cultured cells
Dmso
Ht 29
Mcas
Mcf7
Ovarian cancer
Thymidine
Corresponding Organization :
Other organizations : University of Southern California, Baxter (United States), Stanford University, University of California, Los Angeles, Ontario Institute for Cancer Research, Princess Margaret Cancer Centre, Harvard University
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Variable analysis
independent variables
- Concentration of CFI-400945 (PLK4 inhibitor): 10 nM, 50 nM, 100 nM, or 500 nM
- Unclear from the information provided
- Cell lines: breast cancer (HCC1569, HCC1954, BT549, MDA-MB-415, HCC202, MCF7, T47D, CAL51), ovarian cancer (KOC-7C, SKOV3, HEYC2, COLO704, RMG-1, KK, ES-2, EFO21, MCAS, A2780, OV167, PEA2, TOV-21G, OV90, OV207, Kuramochi, OVISE, OVMANA, PEO6, CaOv-3, OV177, OVCAR3, OVCAR5, OVSAHO, OVTOKO, PEO14), colorectal cancer (HCT-116, HCT-15, DLD-1, COLO 205, COLO320DM, COLO320HSR, NCI-H747, NCI-H716, COLO201, LS1034, SNU-C2B, SNU-C1, LS513, LS411N, ATR-FLOX, LS174t, WiDr, RKO, SK-CO1, HCT-8, LS-180, HT-29, C2Bbe1, SW480), and immortalized breast epithelial (MCF-10–2A, MCF-12A) cell lines
- Cell culture conditions: Cells were grown as described elsewhere (42–44)
- DMSO controls
- None specified
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