Transwell Assay for PC-3 and DU-145 Cell Migration

Migration assays for PC-3 and DU-145 cells were performed following the instructions of the transwell kit purchased from BD Bioscience (Franklin Lakes, NJ, U.S.A.) and as previously described [44 (link)]. PC-3 and DU-145 cells seeded at a concentration of 2 × 10⁴/250 μl into upper compartment of transwell. The lower compartment of transwell was filled with 800 μl complete medium. PC-3 and DU-145 PCa cells were pre-treated with 0, 20, 40 and 80 μM CAPE for 24 h. After appropriate incubation time, total number of migrated cells was counted following standard procedures. Cells were then removed from tissue culture plates with trypsin, and washed once with DMEM serum free medium.

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Tseng J.C., Lin C.Y., Su L.C., Fu H.H., Yang S.D, & Chuu C.P. (2016). CAPE suppresses migration and invasion of prostate cancer cells via activation of non-canonical Wnt signaling. Oncotarget, 7(25), 38010-38024.

Publication 2016

transwell kit

Cells Migration assays Serum Tissue Trypsin

Corresponding Organization : China Medical University

Other organizations : National Tsing Hua University

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Variable analysis

independent variables

CAPE concentration (0, 20, 40, 80 μM)

dependent variables

Total number of migrated cells

control variables

Cell line (PC-3 and DU-145 prostate cancer cells)
Cell seeding density (2 × 10^4 cells/250 μl)
Incubation time (not explicitly mentioned)
Transwell system (BD Bioscience)

controls

Positive control: Not explicitly mentioned
Negative control: 0 μM CAPE treatment

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