Quantifying miR-21 Expression in Gastric Cancer

qRT-PCR was performed for miR-21 as described above. CISH was performed on FFPE tissue sections using a miRCURY LNA microRNA detection kit (Exiqon, details in supplementary materials). For the miR-21 transfection study, 15-PGDH-directed miR-21 (UAGUCAGACUAUUCGAu) was constructed as previously described^{17 (link)}. The miR-21 and scramble mimic were transfected into gastric cancer cells using RNAiMAX (Invitrogen) according to the manufacturer’s instructions.

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Park Y.S., Lee J.H., Jung D.B., Kim H.B., Jung J.H., Pak S., Ryu Y.M., Park H.J., Park Y.Y., Jung H.Y, & Myung S.J. (2018). MicroRNA-21 induces loss of 15-hydroxyprostaglandin dehydrogenase in early gastric tubular adenocarcinoma. Scientific Reports, 8, 17717.

Publication 2018

miRCURY LNA microRNA detection kit RNAiMAX

15 pgdh Cells Cish Gastric cancer Microrna Tissue Transfection

Corresponding Organization :

Other organizations : University of Ulsan, Asan Medical Center, Ulsan College

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Variable analysis

independent variables

MiR-21 transfection
MiR-21 mimic
Scramble mimic

dependent variables

MiR-21 expression (by qRT-PCR)
MiR-21 localization (by CISH)

control variables

FFPE tissue sections
MiRCURY LNA microRNA detection kit (Exiqon)
RNAiMAX (Invitrogen) for transfection

controls

Positive control: Not explicitly mentioned
Negative control: Scramble mimic

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