Anti-SNAP-25, anti-actin, anti-Flag, anti-GST, anti-syntaxin, peroxidase conjugated anti-rabbit IgG and anti-chicken IgG were purchased from Sigma-Aldrich (St. Louis, MO, USA). Anti-SNAP-25pThr138 (Abgent, San Diego, CA, USA), anti-MYPT11-296 [20 (link)], anti-PP1cδ (Millipore, Billerica, MA, USA), anti-GAPDH (Santa Cruz, CA, USA), anti-CPI17pThr38 [22 (link)], horseradish peroxidase-linked anti-mouse IgG (Cell Signalling, Danvers, MA, USA), Clean-Blot IP Detection Reagent (Thermo Scientific, Waltham, MA, USA) and Texas Red-X phalloidin (Life Technologies, Carlsbad, CA, USA) were purchased as indicated. Alexa Fluor 488-conjugated anti-rabbit IgG, Alexa Fluor 546-conjugated anti-mouse IgG, Alexa Fluor 546-conjugated anti-goat IgG and To-Pro-3 iodide were obtained from Molecular Probes (Eugene, OR, USA).
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