Macrocolony Assays for E. coli Biofilms

E. coli macrocolony assays were performed similarly to those in a previous study (83 (link)). Agar plates containing 40 μg/ml Congo red (Sigma-Aldrich, Buchs, Switzerland) and 20 μg/ml Coomassie brilliant blue G (Sigma-Aldrich, Buchs, Switzerland) or 0.01% calcofluor fluorescent brightener 28 (calcofluor; Sigma-Aldrich, Buchs, Switzerland) were spotted with 5 μl ON cultures of E. coli strains and incubated at 28 and 37°C for 7 and 3 days, respectively. LB, LBnoS, RPSM_dil, and ABTCAA agars were used with both staining methods. LB and LBnoS were also used without addition of any dyes.

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Marti R., Schmid M., Kulli S., Schneeberger K., Naskova J., Knøchel S., Ahrens C.H, & Hummerjohann J. (2017). Biofilm Formation Potential of Heat-Resistant Escherichia coli Dairy Isolates and the Complete Genome of Multidrug-Resistant, Heat-Resistant Strain FAM21845. Applied and Environmental Microbiology, 83(15), e00628-17.

Publication 2017

Congo red Coomassie brilliant blue G

Agars Assays Coomassie brilliant blue g Dyes E coli Staining methods Strains

Corresponding Organization :

Other organizations : Federal Food Safety and Veterinary Office, Agroscope, SIB Swiss Institute of Bioinformatics, University of Copenhagen

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Variable analysis

independent variables

Temperature (28°C and 37°C)
Incubation time (7 days and 3 days)

dependent variables

Macrocolony formation of E. coli strains

control variables

Agar plates containing 40 μg/ml Congo red and 20 μg/ml Coomassie brilliant blue G or 0.01% calcofluor fluorescent brightener 28
Media used: LB, LBnoS, RPSM_dil, and ABTCAA

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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