Stills of the actin and microtubule networks in epidermal pavement cells were taken by a Zeiss LSM880 microscope with a Plan-Apochromat 40 x/1.2 W objective and 488-nm argon laser for excitation, videos were taken by an inverted spinning disc confocal microscope (Yokogawa CSU-X1 on a Nikon Ti-E platform, laser box Agilent MLC400, camera Andor Ixon) with 100 x/1,45 O plan apochromatic objective, excitation laser line set at 488 nm, and image interval 1 s as described previously (Rosero et al., 2016 (link)). Cytoskeleton bundling and density were quantified as described previously (Higaki et al., 2010 (link)) with minor modifications (Rosero et al., 2013 (link); Rosero et al., 2019 (link)).
Cytoskeletal dynamics measurements were done in two biological replicates: with at least 40 cells from at least 20 videos analyzed using the QuACK method (Cvrčková and Oulehlová, 2017 (link)).
Actin networks in developing trichromes were visualized using an inverted spinning disc confocal microscope (Zeiss Axio Observer 7 microscope with a vertical stage equipped with a Yokogawa CSU-W1 spinning disk unit and Photometrics Prime 95B camera) with Zeiss Plan Apochromat 40 x/1.2 W objective and 488-nm laser excitation line.
Cytoskeletal dynamics measurements were done in two biological replicates: with at least 40 cells from at least 20 videos analyzed using the QuACK method (Cvrčková and Oulehlová, 2017 (link)).
Actin networks in developing trichromes were visualized using an inverted spinning disc confocal microscope (Zeiss Axio Observer 7 microscope with a vertical stage equipped with a Yokogawa CSU-W1 spinning disk unit and Photometrics Prime 95B camera) with Zeiss Plan Apochromat 40 x/1.2 W objective and 488-nm laser excitation line.
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