Immunofluorescence Microscopy for Tubulin

Immunofluorescence was carried out as described in [35 (link)]. Anti-tubulin (Abcam) and anti–rat FITC (Jackson ImmunoResearch) antibodies were used at 1:250. Microscope preparations were imaged using a DM6000 microscope (Leica) equipped with a 100x/1.40 NA oil immersion objective lens, A4, L5, and TX2 filters, and a DF350 digital charge-coupled device camera (Leica). Pictures were processed with LAS AF (Leica) and ImageJ (http://rsbweb.nih.gov/ij/) software.

Free full text: Click here

Muñoz-Barrera M., Aguilar I, & Monje-Casas F. (2015). Dispensability of the SAC Depends on the Time Window Required by Aurora B to Ensure Chromosome Biorientation. PLoS ONE, 10(12), e0144972.

Publication 2015

Anti-tubulin anti–rat FITC DM6000 microscope LAS AF

Antibodies Camera Device Fitc Immersion Immunofluorescence Lens Microscope Tubulin

Corresponding Organization : Universidad de Sevilla

Top 5 similar protocols

Variable analysis

independent variables

Antibody used: Anti-tubulin (Abcam) and anti–rat FITC (Jackson ImmunoResearch) antibodies

dependent variables

Microscope images of the samples

control variables

Antibody concentration: 1:250
Microscope equipment: DM6000 microscope (Leica) equipped with a 100x/1.40 NA oil immersion objective lens, A4, L5, and TX2 filters, and a DF350 digital charge-coupled device camera (Leica)
Image processing software: LAS AF (Leica) and ImageJ

controls

No positive or negative controls were explicitly mentioned in the provided information.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!