Evaluating Keratinocyte Migration Dynamics

Monolayer wounding assays were used to evaluate migration of keratinocytes as described before [95] (link). Briefly, NHK cells were grown to confluence in ibidi μ-dishes containing culture inserts. Cells were incubated in Keratinocyte SFM® medium (with rhEGF, BPE and penicillin-streptomycin) containing 1 μg/mL TE or 10 ng/mL HGF. Live cell imaging migration was recorded in the PFS system on a Nikon Eclipse Ti microscope with a digital sight DS-QiMc (Nikon Instruments Inc., Tokyo, Japan), coupled to an ibidi-heating chamber. The migratory activity was measured by calculating the percentage of closed areas.

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Ebeling S., Naumann K., Pollok S., Wardecki T., Vidal-y-Sy S., Nascimento J.M., Boerries M., Schmidt G., Brandner J.M, & Merfort I. (2014). From a Traditional Medicinal Plant to a Rational Drug: Understanding the Clinically Proven Wound Healing Efficacy of Birch Bark Extract. PLoS ONE, 9(1), e86147.

Publication 2014

DS-QiMc

Assays Cell migration Cells Dishes Keratinocyte Microscope Penicillin Sight Streptomycin

Corresponding Organization : University of Freiburg

Other organizations : Universität Hamburg, University Medical Center Hamburg-Eppendorf, German Cancer Research Center

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Variable analysis

independent variables

TE (1 μg/mL)
HGF (10 ng/mL)

dependent variables

Migratory activity (percentage of closed areas)

control variables

NHK cells
Keratinocyte SFM® medium (with rhEGF, BPE and penicillin-streptomycin)
Monolayer wounding assays
Live cell imaging migration recorded in the PFS system on a Nikon Eclipse Ti microscope with a digital sight DS-QiMc (Nikon Instruments Inc., Tokyo, Japan), coupled to an ibidi-heating chamber

controls

Positive control: Not specified
Negative control: Not specified

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