Chitosan-Doxorubicin Nanocarrier Synthesis

Chitosan with average molecular weight (MW) of 1KDa, 3KDa, 10KDa, 50KDa and 300KDa was purchased from Aoxing Biotechnology Co. Ltd.. Doxorubicin (Dox), fluorescein (Flu) were purchased from Sigma-Aldrich. RPMI-1640 medium, thiazolyl blue (MTT), fetal bovine serum and trypsin were purchased from Keygen Biotech. Inc.. Octanal, sodium borohydride (NaBH4) were purchased from China National Medicine Corporation Ltd.. MPA (MW: 995) was prepared in our laboratory following Achilefu's report [33 (link), 34 (link)]. All other solvents and reagents used in this study were certified analytical reagent grade.

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Zhang M., Ma Y., Wang Z., Han Z., Gao W, & Gu Y. (2017). Optimizing molecular weight of octyl chitosan as drug carrier for improving tumor therapeutic efficacy. Oncotarget, 8(38), 64237-64249.

Publication 2017

RPMI-1640 medium trypsin

Chitosan Doxorubicin Fetal bovine serum Fluorescein Medicine Octanal Sodium borohydride Solvents Thiazolyl blue Trypsin

Corresponding Organization :

Other organizations : China Pharmaceutical University

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Variable analysis

independent variables

Chitosan with average molecular weight (MW) of 1KDa, 3KDa, 10KDa, 50KDa and 300KDa

dependent variables

Not explicitly mentioned

control variables

Doxorubicin (Dox), fluorescein (Flu), RPMI-1640 medium, thiazolyl blue (MTT), fetal bovine serum, trypsin, octanal, sodium borohydride (NaBH4), MPA (MW: 995)

controls

Positive control: Not specified
Negative control: Not specified

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