Nanoliposome Morphology Evaluation via TEM

The morphology of nanoliposomes was monitored via TEM using a negative staining method as previously described [30 (link)]. In brief, the liposomal formulation was diluted in distilled water (1:10 ratio) and then mixed with a 2% ammonium molybdate solution with a ratio of 1:1. The mixture was reserved at room temperature for 3 min. Then, one drop was placed and dried on a Formvar carbon-coated copper grid (200 mesh, 3 mm diameter HF 36). Then, the morphology of the nanoliposomes was examined using a Philips CM20 TEM equipped with an Olympus TEM CCD camera at 200 kV.

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Li J., Elkhoury K., Barbieux C., Linder M., Grandemange S., Tamayol A., Francius G, & Arab-Tehrany E. (2020). Effects of Bioactive Marine-Derived Liposomes on Two Human Breast Cancer Cell Lines. Marine Drugs, 18(4), 211.

Publication 2020

CM20 TEM TEM CCD camera

Ammonium molybdate Carbon Copper Formvar Liposomal Mm 36 Negative staining method

Corresponding Organization :

Other organizations : Centre National de la Recherche Scientifique, Université de Lorraine, University of Connecticut, Laboratoire de Chimie Physique et Microbiologie pour l'Environnement

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Variable analysis

independent variables

Dilution ratio of liposomal formulation in distilled water
Ratio of diluted liposomal formulation to 2% ammonium molybdate solution

dependent variables

Morphology of nanoliposomes

control variables

Formvar carbon-coated copper grid (200 mesh, 3 mm diameter HF 36)
Philips CM20 TEM equipped with an Olympus TEM CCD camera at 200 kV

controls

Negative control: None mentioned
Positive control: None mentioned

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