Pharmacological Modulation of Autism-Like Behaviors

After 14 days from OA induction, CTR and MIA mice were divided into three experimental groups (n = 14/each) and started the treatment with either drugs or vehicle. In detail, both CTR and MIA mice were treated with vehicle (saline solution, 10 µL/g), with the non-peptidic antagonist of the PK system PC1 (s.c., 150 µg/kg, twice a day) [37 (link),42 (link),43 (link)] or with the NSAID diclofenac (i.p., 10 mg/kg, once a day, Novartis, Milan, Italy) [44 (link)]. All drugs were freshly prepared, and each treatment was daily (once/twice) administered for 14 days (from day 14 until day 27). Mice were sacrificed the day after the last drug administration (day 28).