ChIP-seq and Motif Analysis of GATA4 and FOXC1

ChIP was performed as previously described for U2OS cells [6 (link)] and Flag-biotin-tagged GATA4 [5 (link)]. ChIP-sequencing was previously described [8 (link)]. Motif analysis was performed using Meme Suite [25 (link)]. FOXC1 was immunoprecipitated with a goat polyclonal antibody (Abcam, ab5079). The specific primers for SYBR Green assays used for qPCR are listed in Supplemental Table S1. All data were compared to the percent input and a negative control region [8 (link)].

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Suthon S., Lin J., Perkins R.S., Miranda-Carboni G.A, & Krum S.A. (2023). Regulation and Function of FOXC1 in Osteoblasts. Journal of Developmental Biology, 11(3), 38.

Publication 2023

ab5079

Antibody Assays Biotin Cells Chip Goat Primers Sybr green

Corresponding Organization : Center for Cancer Research

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Variable analysis

independent variables

ChIP was performed as previously described for U2OS cells [6 (link)]
Flag-biotin-tagged GATA4 [5 (link)]
FOXC1 was immunoprecipitated with a goat polyclonal antibody (Abcam, ab5079)

dependent variables

ChIP-sequencing was previously described [8 (link)]
Motif analysis was performed using Meme Suite [25 (link)]

control variables

The specific primers for SYBR Green assays used for qPCR are listed in Supplemental Table S1
All data were compared to the percent input and a negative control region [8 (link)]

negative controls

A negative control region [8 (link)]

Annotations

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