β-Hydroxybutyrate (Sigma–Aldrich, St. Louis, MO, USA, working concentration, 2 mM) was added to the standard food media. Three-day-old esg > GFP, wild type flies, 38-day-old esg > GFP, wild type flies, and 3-day-old esg > GFP + Catn1, Catn1/+ flies were fed 2 mM β-HB in standard media for seven days at 25 °C [18 (link),22 (link)]. Every two days, flies were transferred to fresh food vials containing β-HB.
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