Fluorescence Microscopy Imaging Protocol

Fluorescence microscopy was carried out as described previously [15 (link),60 (link)] using the following filter cubes: F400 (excitation: 390–410 nm; dichroic mirror: 505 nm; emission: 510–550 nm), F425 (excitation: 422–432 nm; dichroic mirror: 600 nm; emission: 610 long pass), F480 (excitation: 470–495 nm; dichroic mirror: 505 nm; emission: 510–550 nm), and F565 (excitation: 545–580 nm; dichroic mirror: 600 nm; emission: 610 nm long pass). The cells were seeded and imaged in FluoroDish cell culture dishes (World Precision Instruments, Hertfordshire, UK; FD-35) precoated with 25 μg/mL polyethyleneimine (MP Biomedicals, 195444) [46 (link)], and cellSens Dimension software (version 2.1) (Olympus Belgium) was used for image acquisition and analysis.

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Costa C.F., Lismont C., Chornyi S., Li H., Hussein M.A., Waterham H.R, & Fransen M. (2023). Functional Analysis of GSTK1 in Peroxisomal Redox Homeostasis in HEK-293 Cells. Antioxidants, 12(6), 1236.

Publication 2023

FluoroDish cell culture dishes polyethyleneimine cellSens Dimension software (version 2.1)

Cell culture Cells Cubes Dishes Fluorescence microscopy Polyethyleneimine

Corresponding Organization :

Other organizations : KU Leuven, University Medical Center, University of Amsterdam, Assiut University

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Variable analysis

independent variables

Fluorescence microscopy filter cubes (F400, F425, F480, F565)

dependent variables

Microscopy images

control variables

Cells seeded and imaged in FluoroDish cell culture dishes precoated with 25 μg/mL polyethyleneimine
Image acquisition and analysis using cellSens Dimension software (version 2.1)

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

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