Isolation of Cerebellar Endothelial Cells

At the specified time points, cerebellar endothelial cells were isolated by mechanical and enzymatic digestion followed by separation using magnetic-activated cell sorting by anti-CD31/PECAM1-conjugated magnetic beads (MACS MS system, Miltenyi Biotec), as previously described^{2 (link)}.

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Ren A.A., Snellings D.A., Su S.Y., Hong C.C., Castro M., Tang A.T., Detter M.R., Hobson N., Girard R., Romanos S., Lightle R., Moore T., Shenkar R., Benavides C., Beaman M.M., Mueller-Fielitz H., Chen M., Mericko P., Yang J., Sung D.C., Lawton M.T., Ruppert M., Schwaninger M., Körbelin J., Potente M., Awad I.A., Marchuk D.A, & Kahn M.L. (2021). PIK3CA and CCM mutations fuel cavernomas through a cancer-like mechanism. Nature, 594(7862), 271-276.

Publication 2021

MACS MS system

Cerebellar Digestion Endothelial cells Enzymatic Pecam1

Corresponding Organization :

Other organizations : University of Pennsylvania, Duke University, Max Planck Institute for Heart and Lung Research, University of Chicago, University of Lübeck, Barrow Neurological Institute, West Virginia University, Universität Hamburg, University Medical Center Hamburg-Eppendorf, Humboldt-Universität zu Berlin, Charité - Universitätsmedizin Berlin, Freie Universität Berlin

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Variable analysis

independent variables

Time points when cerebellar endothelial cells were isolated

dependent variables

Isolated cerebellar endothelial cells

control variables

Mechanical and enzymatic digestion process
Separation using magnetic-activated cell sorting by anti-CD31/PECAM1-conjugated magnetic beads (MACS MS system, Miltenyi Biotec)

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