Mycelial Sterol Extraction and Analysis

Samples for sterol extraction were prepared as that for RNA extraction described above. Mycelial samples were heat-dried at 80 °C and then ground into fine powder using a mortar and pestle. About 10 mg of mycelial powder was used for sterol extraction in Agilent vials (2 mL). Extraction and chemical analysis of sterols and KTC were performed as previously described [36 (link)] using ergosterol (J&K Scientific, Beijing, China) and KTC standards. The statistical analysis was conducted as previously described [36 (link)].

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Zhou M., Hu C., Yin Y., Wang J., Ye S., Yu Y., Sun X, & Li S. (2022). Experimental Evolution of Multidrug Resistance in Neurospora crassa under Antifungal Azole Stress. Journal of Fungi, 8(2), 198.

Publication 2022

Agilent vials ergosterol

Ergosterol Mycelial Powder Sterol Sterols

Corresponding Organization : Chinese Academy of Sciences

Other organizations : Tianjin University of Commerce

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Variable analysis

independent variables

Mycelial samples preparation method (heat-drying at 80 °C and grinding into fine powder)

dependent variables

Sterol content
KTC content

control variables

Sample weight (about 10 mg of mycelial powder)

controls

Ergosterol standard
KTC standard

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