Proliferation, Invasion, and Migration Assays in BrCa Cells

Cell proliferation, invasion, and migration assays were performed in BrCa cells. Briefly, cell proliferation was assessed using XTT assays (Sigma-Aldrich, St. Louis, MO, USA); invasion was evaluated using Matrigel chamber assays with Corning BioCoat Matrigel (Corning, New York, NY, USA); and migration was examined using chamber assays with Corning BioCoat cell culture chambers (Corning). Details of the procedures are included in our previous studies [29 (link),30 (link)].

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Mitsueda R., Toda H., Shinden Y., Fukuda K., Yasudome R., Kato M., Kikkawa N., Ohtsuka T., Nakajo A, & Seki N. (2023). Oncogenic Targets Regulated by Tumor-Suppressive miR-30c-1-3p and miR-30c-2-3p: TRIP13 Facilitates Cancer Cell Aggressiveness in Breast Cancer. Cancers, 15(16), 4189.

Publication 2023

XTT assays Corning BioCoat Matrigel

Assays Cell culture Cell proliferation Cells Matrigel Migration assays

Corresponding Organization : Chiba University

Other organizations : Kagoshima University

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Variable analysis

independent variables

Not explicitly mentioned

dependent variables

Cell proliferation
Cell invasion
Cell migration

control variables

Not explicitly mentioned

controls

Positive control: Not specified
Negative control: Not specified

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