We used brain tissues from humans, HIP, WT and AKO rats, and isolated brain capillaries from HIP, WT and AKO rats. Brain tissue was processed as previously29 (link) described. Brain capillaries were isolated (as described above) and then plated on laminin (23017-015, Gibco, MA) coated chambered coverglass for 1-hour, to allow time to attach.
Antibodies against human amylin (1:200, SC-377530, Santa Cruz, TX), collagen IV (1:500, SAB4200500, Sigma, MO), caveolin1 (1:1,000, sc-894, Santa Cruz, TX) and APOE (1:200, ab183597, Abcam, MA) were the primary antibodies. Alexa Fluor 488 conjugated anti-mouse IgG (A11029, Invitrogen, MA) and Alexa Fluor 568 conjugated anti-rabbit IgG (A11036, Invitrogen, MA) were the secondary antibodies.
Antibodies against human amylin (1:200, SC-377530, Santa Cruz, TX), collagen IV (1:500, SAB4200500, Sigma, MO), caveolin1 (1:1,000, sc-894, Santa Cruz, TX) and APOE (1:200, ab183597, Abcam, MA) were the primary antibodies. Alexa Fluor 488 conjugated anti-mouse IgG (A11029, Invitrogen, MA) and Alexa Fluor 568 conjugated anti-rabbit IgG (A11036, Invitrogen, MA) were the secondary antibodies.
