Optimized Mycoplasma gallisepticum Culture

Mycoplasma gallisepticum strain S6 was obtained from the Chinese Veterinary Microorganism Culture Collection Center (Beijing, China). Culture media consisted of M. gallisepticum artificial medium base (Qingdao Hope Biological Technology) containing nicotinamide adenine dinucleotide and cysteine and swine serum (Guangzhou Ruite Biological) as previously described (3 (link)). Tilmicosin phosphate was a kind gift of Eastern Along Pharmaceuticals (Guangdong, China). Acetonitrile, formic acid, methanol (high-performance liquid chromatography grade), and remaining analytical grade reagents were purchased from Guangzhou Chemical Reagent.

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Zhang N., Zhou M., Yan X., Liu J., Yuan S., Yang H., Ding H., Zhang D, & Bai Y. (2022). Pharmacokinetic and Pharmacodynamic integration of tilmicosin against Mycoplasma gallisepticum in the target infection site in chickens. Frontiers in Veterinary Science, 9, 952599.

Publication 2022

Acetonitrile formic acid methanol

Acetonitrile Biological Chinese Cysteine Formic acid High performance liquid chromatography Methanol Mycoplasma gallisepticum Nicotinamide adenine dinucleotide Pharmaceuticals Phosphate Serum Strain Swine Tilmicosin

Corresponding Organization : Foshan University

Other organizations : South China Agricultural University

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Variable analysis

independent variables

Tilmicosin phosphate

dependent variables

Not explicitly mentioned

control variables

Culture media consisting of M. gallisepticum artificial medium base containing nicotinamide adenine dinucleotide and cysteine, and swine serum
Remaining analytical grade reagents (acetonitrile, formic acid, methanol)

controls

Positive control: Not mentioned
Negative control: Not mentioned

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