A microfluidic-based qPCR dynamic array was used for the RNA expression analysis in samples derived from the Andalusian Public Health System Biobank cohort of patients.133 (link)–135 (link) Specific primers for human transcripts were designed with Primer-BLAST tool (https://www.ncbi.nlm.nih.gov/tools/primer-blast/) and synthesized by Sigma-Aldrich (Table S2). Preamplification, exonuclease treatment and qPCR dynamic array based on microfluidic technology were implemented using the Biomark System (Fluidigm) by following the manufacturer’s instructions.136 (link) mRNA copy number of the transcripts analyzed were adjusted by normalization factor, calculated with the expression levels of ACTB, GAPDH and HPRT using geNorm 3.3 software.137 (link) Pearson correlation analyses were computed on mRNA expression levels and reported the values of the correlation coefficient (r) and two-tailed p values. The coefficient of determination (R2) was calculated from the Pearson correlation coefficient, and the best-fit line was plotted for each correlation.
Lachiondo-Ortega S., Rejano-Gordillo C.M., Simon J., Lopitz-Otsoa F., Delgado T.C., Mazan-Mamczarz K., Goikoetxea-Usandizaga N., Estefanía Zapata-Pavas L., Garcıía-del Río A., Guerra P., Peña-Sanfélix P., Hermán-Sánchez N., Al-Abdulla R., Fernandez-Rodríguez C., Azkargorta M., Velázquez-Cruz A., Guyon J., Martín C., Zalamea J.D., Egia-Mendikute L., Sanz-Parra A., Serrano-Maciá M., González-Recio I., Gonzalez-Lopez M., Martínez-Cruz L.A., Pontisso P., Aransay A.M., Barrio R., Sutherland J.D., Abrescia N.G., Elortza F., Lujambio A., Banales J.M., Luque R.M., Gahete M.D., Palazón A., Avila M.A., Marin J.J., De S., Daubon T., Díaz-Quintana A., Díaz-Moreno I., Gorospe M., Rodríguez M.S, & Martínez-Chantar M.L. (2024). SUMOylation controls Hu antigen R posttranscriptional activity in liver cancer. Cell reports, 43(3), 113924.
Corresponding Organization : Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas
Other organizations :
Universidad de Extremadura, Consejo Superior de Investigaciones Científicas, University of the Basque Country, Institute on Aging, National Institutes of Health, National Institute on Aging, University of Padua, University of Córdoba, Instituto Maimónides de Investigación Biomédica de Córdoba, Universitätsmedizin Greifswald, Universitat de Miguel Hernández d'Elx, Instituto de Investigaciones Químicas, Universidad de Sevilla, Centro de Investigaciones Científicas Isla de la Cartuja, Centre Hospitalier Universitaire de Bordeaux, Inserm, Université de Bordeaux, Ikerbasque, Icahn School of Medicine at Mount Sinai, Biogipuzkoa Health Research Institute, Universidad de Navarra, Navarre Institute of Health Research, Instituto de Investigación Biomédica de Salamanca, Universidad de Salamanca, Centre National de la Recherche Scientifique, Université Toulouse III - Paul Sabatier, Laboratoire de Chimie de Coordination
Samples derived from the Andalusian Public Health System Biobank cohort of patients
dependent variables
RNA expression levels
MRNA copy number of the transcripts analyzed
control variables
Expression levels of ACTB, GAPDH and HPRT used for normalization
controls
Positive control: None specified
Negative control: None specified
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